The G-protein γ subunit of Phytophthora infestans is involved in sporangial development.
Identifieur interne : 000648 ( Main/Exploration ); précédent : 000647; suivant : 000649The G-protein γ subunit of Phytophthora infestans is involved in sporangial development.
Auteurs : Johan Van Den Hoogen [Pays-Bas] ; Natalie Verbeek-De Kruif [Pays-Bas] ; Francine Govers [Pays-Bas]Source :
- Fungal genetics and biology : FG & B [ 1096-0937 ] ; 2018.
Descripteurs français
- KwdFr :
- Interférence par ARN (MeSH), Mycelium (métabolisme), Phytophthora infestans (croissance et développement), Phytophthora infestans (génétique), Sous-unités gamma des protéines G (génétique), Sous-unités gamma des protéines G (physiologie), Sporanges (croissance et développement), Sporanges (génétique), Spores (métabolisme), Séquence conservée (MeSH).
- MESH :
- croissance et développement : Phytophthora infestans, Sporanges.
- génétique : Phytophthora infestans, Sous-unités gamma des protéines G, Sporanges.
- métabolisme : Mycelium, Spores.
- physiologie : Sous-unités gamma des protéines G.
- Interférence par ARN, Séquence conservée.
English descriptors
- KwdEn :
- Conserved Sequence (MeSH), GTP-Binding Protein gamma Subunits (genetics), GTP-Binding Protein gamma Subunits (physiology), Mycelium (metabolism), Phytophthora infestans (genetics), Phytophthora infestans (growth & development), RNA Interference (MeSH), Sporangia (genetics), Sporangia (growth & development), Spores (metabolism).
- MESH :
- chemical , genetics : GTP-Binding Protein gamma Subunits.
- chemical , physiology : GTP-Binding Protein gamma Subunits.
- genetics : Phytophthora infestans, Sporangia.
- growth & development : Phytophthora infestans, Sporangia.
- metabolism : Mycelium, Spores.
- Conserved Sequence, RNA Interference.
Abstract
The oomycete Phytophthora infestans is a notorious plant pathogen with potato and tomato as its primary hosts. Previous research showed that the heterotrimeric G-protein subunits Gα and Gβ have a role in zoospore motility and virulence, and sporangial development, respectively. Here, we present analyses of the gene encoding a Gγ subunit in P. infestans, Pigpg1. The overall similarity of PiGPG1 with non-oomycete Gγ subunits is low, with only the most conserved amino acids maintained, but similarity with its homologs in other oomycetes is high. Pigpg1 is expressed in all life stages and shows a similar expression profile as the gene encoding the Gβ subunit, Pigpb1. To elucidate its function, transformants were generated in which Pigpg1 is silenced or overexpressed and their phenotypes were analyzed. Pigpg1-silenced lines produce less sporangia, which are malformed. Altogether, the results show that PiGPG1 is crucial for proper sporangia development and zoosporogenesis. PiGPG1 is a functional Gγ, and likely forms a dimer with PiGPB1 that mediates signaling.
DOI: 10.1016/j.fgb.2018.04.012
PubMed: 29704555
Affiliations:
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Electronic address: francine.govers@wur.nl.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Laboratory of Phytopathology, Wageningen University, Droevendaalsesteeg 1, 6808 PB Wageningen</wicri:regionArea><orgName type="university">Université de Wageningue</orgName><placeName><settlement type="city">Wageningue</settlement><region nuts="2">Gueldre (province)</region></placeName></affiliation></author></analytic><series><title level="j">Fungal genetics and biology : FG & B</title><idno type="eISSN">1096-0937</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Conserved Sequence (MeSH)</term><term>GTP-Binding Protein gamma Subunits (genetics)</term><term>GTP-Binding Protein gamma Subunits (physiology)</term><term>Mycelium (metabolism)</term><term>Phytophthora infestans (genetics)</term><term>Phytophthora infestans (growth & development)</term><term>RNA Interference (MeSH)</term><term>Sporangia (genetics)</term><term>Sporangia (growth & development)</term><term>Spores (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Interférence par ARN (MeSH)</term><term>Mycelium (métabolisme)</term><term>Phytophthora infestans (croissance et développement)</term><term>Phytophthora infestans (génétique)</term><term>Sous-unités gamma des protéines G (génétique)</term><term>Sous-unités gamma des protéines G (physiologie)</term><term>Sporanges (croissance et développement)</term><term>Sporanges (génétique)</term><term>Spores (métabolisme)</term><term>Séquence conservée (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>GTP-Binding Protein gamma Subunits</term></keywords><keywords scheme="MESH" type="chemical" qualifier="physiology" xml:lang="en"><term>GTP-Binding Protein gamma Subunits</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Phytophthora infestans</term><term>Sporanges</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Phytophthora infestans</term><term>Sporangia</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Phytophthora infestans</term><term>Sporangia</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Phytophthora infestans</term><term>Sous-unités gamma des protéines G</term><term>Sporanges</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Mycelium</term><term>Spores</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Mycelium</term><term>Spores</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Sous-unités gamma des protéines G</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Conserved Sequence</term><term>RNA Interference</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Interférence par ARN</term><term>Séquence conservée</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The oomycete Phytophthora infestans is a notorious plant pathogen with potato and tomato as its primary hosts. Previous research showed that the heterotrimeric G-protein subunits Gα and Gβ have a role in zoospore motility and virulence, and sporangial development, respectively. Here, we present analyses of the gene encoding a Gγ subunit in P. infestans, Pigpg1. The overall similarity of PiGPG1 with non-oomycete Gγ subunits is low, with only the most conserved amino acids maintained, but similarity with its homologs in other oomycetes is high. Pigpg1 is expressed in all life stages and shows a similar expression profile as the gene encoding the Gβ subunit, Pigpb1. To elucidate its function, transformants were generated in which Pigpg1 is silenced or overexpressed and their phenotypes were analyzed. Pigpg1-silenced lines produce less sporangia, which are malformed. Altogether, the results show that PiGPG1 is crucial for proper sporangia development and zoosporogenesis. PiGPG1 is a functional Gγ, and likely forms a dimer with PiGPB1 that mediates signaling.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">29704555</PMID><DateCompleted><Year>2019</Year><Month>02</Month><Day>21</Day></DateCompleted><DateRevised><Year>2019</Year><Month>02</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1096-0937</ISSN><JournalIssue CitedMedium="Internet"><Volume>116</Volume><PubDate><Year>2018</Year><Month>07</Month></PubDate></JournalIssue><Title>Fungal genetics and biology : FG & B</Title><ISOAbbreviation>Fungal Genet Biol</ISOAbbreviation></Journal><ArticleTitle>The G-protein γ subunit of Phytophthora infestans is involved in sporangial development.</ArticleTitle><Pagination><MedlinePgn>73-82</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S1087-1845(18)30071-9</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.fgb.2018.04.012</ELocationID><Abstract><AbstractText>The oomycete Phytophthora infestans is a notorious plant pathogen with potato and tomato as its primary hosts. Previous research showed that the heterotrimeric G-protein subunits Gα and Gβ have a role in zoospore motility and virulence, and sporangial development, respectively. Here, we present analyses of the gene encoding a Gγ subunit in P. infestans, Pigpg1. The overall similarity of PiGPG1 with non-oomycete Gγ subunits is low, with only the most conserved amino acids maintained, but similarity with its homologs in other oomycetes is high. Pigpg1 is expressed in all life stages and shows a similar expression profile as the gene encoding the Gβ subunit, Pigpb1. To elucidate its function, transformants were generated in which Pigpg1 is silenced or overexpressed and their phenotypes were analyzed. Pigpg1-silenced lines produce less sporangia, which are malformed. Altogether, the results show that PiGPG1 is crucial for proper sporangia development and zoosporogenesis. PiGPG1 is a functional Gγ, and likely forms a dimer with PiGPB1 that mediates signaling.</AbstractText><CopyrightInformation>Copyright © 2018 The Author(s). Published by Elsevier Inc. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>van den Hoogen</LastName><ForeName>Johan</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Laboratory of Phytopathology, Wageningen University, Droevendaalsesteeg 1, 6808 PB Wageningen, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Verbeek-de Kruif</LastName><ForeName>Natalie</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Laboratory of Phytopathology, Wageningen University, Droevendaalsesteeg 1, 6808 PB Wageningen, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Govers</LastName><ForeName>Francine</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Laboratory of Phytopathology, Wageningen University, Droevendaalsesteeg 1, 6808 PB Wageningen, The Netherlands. Electronic address: francine.govers@wur.nl.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>04</Month><Day>25</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Fungal Genet Biol</MedlineTA><NlmUniqueID>9607601</NlmUniqueID><ISSNLinking>1087-1845</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D044388">GTP-Binding Protein gamma Subunits</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D017124" MajorTopicYN="N">Conserved Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D044388" MajorTopicYN="N">GTP-Binding Protein gamma Subunits</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D025282" MajorTopicYN="N">Mycelium</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D055750" MajorTopicYN="N">Phytophthora infestans</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D034622" MajorTopicYN="N">RNA Interference</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D058432" MajorTopicYN="N">Sporangia</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013170" MajorTopicYN="N">Spores</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">G-protein subunit</Keyword><Keyword MajorTopicYN="Y">GPCR</Keyword><Keyword MajorTopicYN="Y">Oomycetes</Keyword><Keyword MajorTopicYN="Y">Phytophthora</Keyword><Keyword MajorTopicYN="Y">Signaling</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year><Month>03</Month><Day>06</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2018</Year><Month>04</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2018</Year><Month>04</Month><Day>21</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>4</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>2</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>4</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">29704555</ArticleId><ArticleId IdType="pii">S1087-1845(18)30071-9</ArticleId><ArticleId IdType="doi">10.1016/j.fgb.2018.04.012</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Pays-Bas</li></country><region><li>Gueldre (province)</li></region><settlement><li>Wageningue</li></settlement><orgName><li>Université de Wageningue</li></orgName></list><tree><country name="Pays-Bas"><region name="Gueldre (province)"><name sortKey="Van Den Hoogen, Johan" sort="Van Den Hoogen, Johan" uniqKey="Van Den Hoogen J" first="Johan" last="Van Den Hoogen">Johan Van Den Hoogen</name></region><name sortKey="Govers, Francine" sort="Govers, Francine" uniqKey="Govers F" first="Francine" last="Govers">Francine Govers</name><name sortKey="Verbeek De Kruif, Natalie" sort="Verbeek De Kruif, Natalie" uniqKey="Verbeek De Kruif N" first="Natalie" last="Verbeek-De Kruif">Natalie Verbeek-De Kruif</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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